生态学杂志2012，Vol.31Issue(10)：2619-2624,6.
盐度胁迫对尼罗罗非鱼鳃氯细胞调节变化的影响
Effects of salinity stress on the adjustment of branchial chloride cells in Oreochromis niloticus
摘要
Abstract
By the methods of scanning electron micrograph and immunohistochemistry, this paper studied the distribution of epithelial chloride cells ( CC ) in Nile tilapia (Oreochromis niloticus) gills and the effects of different salinity (0, 10, 20, and 30) stress on the CC number and morphology. The surface scanning of the gills showed the epithelial chloride cells were distributed close to the inter-lamellar regions of gill filaments. According to the apical size, the CC could be divided into three subtypes, i.e. , subtype I (>6. 5 |xm) , subtype II (3.2-6.5 (im) , and subtype HI ( <3. 2 |xm). The CC number in the gills at different salinities was in the sequence of salinity 10 < salinity 20 < salinity 0 < salinity 30. When the Nile tilapia transferred to the waters from salinity 0 to salinity 10, the total number of the CC in gills decreased, mainly due to the significant decrease of the subtype I cells. The CC number at salinity 20 was higher than that at salinity 10, but the difference was not significant. The CC number at salinity 30 increased significantly with the increase of subtype HI cells. Immunohistochemical staining further confirmed that at different salinities, the Na+-K+-ATPase immunoreactive (NKA-IR) cells were all distributed in the inter-lamellar region of the gill filaments. It was suggested that Nile tilapia could adapt to the salinity change of the environment through changing the quantity and morphological structure of branchial chloride cells, and the subtypes I and HI cells could play a key role for the os-moregulation in the waters with low and high salinity, respectively.关键词
尼罗罗非鱼/盐度胁迫/鳃/氯细胞/Na+-K+-ATPaseKey words
Oreochromis niloticus/ salinity stress/ gill/ chloride cell/ Na+-K+-ATPase.分类
农业科技引用本文复制引用
庄青青,赵金良,赵丽慧,苌建菊..盐度胁迫对尼罗罗非鱼鳃氯细胞调节变化的影响[J].生态学杂志,2012,31(10):2619-2624,6.基金项目
现代农业产业技术体系专项(CARS-49-4B)资助. （CARS-49-4B）
