生物加工过程2012，Vol.10Issue(5)：28-33,6.DOI:10.3969/j.issn.1672-3678.2012.05.006
废弃活性污泥中产聚羟基脂肪酸酯菌株Bacillus sp.PB-3的发酵条件优化
Optimization of PHAs production by Bacillus sp.PB-3 from waste activated sludge
摘要
Abstract
A high-yield strain of PHAs, Bacillus sp. PB-3 was isolated from waste activated sludge by Nile red staining and fluorescence microscopy screening. Gas chromatography analysis indicated that the intracellular production of the strain was polyhydroxybutyrate ( PHB). Furthermore, medium composition and fermentation factors were investigated and optimized. The optimal carbon and nitrogen sources were glucose(12 g/L) and beef paste(2 g/L) , respectively. The optimal cultivation conditions were pH 7. 5 , 37 ℃ for 48 h, and 80 mL medium at an agitation rate of 200 r/min. Under the fermentation conditions, the yield of PHB was raised to 32. 09% (w/w) , 30% higher than that before optimized.关键词
聚羟基脂肪酸酯/聚羟基丁酸酯/筛选/发酵Key words
PHAs/PHB/screen/fermentation分类
生物科学引用本文复制引用
郝大可,李强,韩省,王佳佳,罗琰,宫恺..废弃活性污泥中产聚羟基脂肪酸酯菌株Bacillus sp.PB-3的发酵条件优化[J].生物加工过程,2012,10(5):28-33,6.基金项目
国家自然科学基金资助项目(31100088) （31100088）
公安部应用创新计划资助项目(2010YYCXSDST054) （2010YYCXSDST054）
