生物加工过程2012，Vol.10Issue(5)：34-38,5.DOI:10.3969/j.issn.1672-3678.2012.05.007
花生四烯酸油脂高产菌株的选育
Breeding of Mortierella alpina for high-yield arachidonic acid-rich oil production
摘要
Abstract
Mortierella alpina ME-1 was mutated with UV-LiCl and screened by selecting plates containing octyl gallate. The ARA-rich oil productivity of mutant R807 was much better than that of the original strain. In the mutant, the content of C18 fatty acids was less than the original strain with a 40% ARA. The dry cell weight, the lipid yield, the ratio of ARA in total fatty acids, and the ARA yield reached 39. 2 g/L, 16. 3 g/L, 41. 72% , and 6. 81 g/L, respectively. They were 22. 9% , 3. 2% , 35. 1% , and 39. 8% , higher than that in the original strain. Furthermore, there were no obvious changes in the production after transferring continuously.关键词
花生四烯酸/高山被孢霉/诱变/没食子酸辛酯Key words
arachidonic acid/Mortierella alpina/mutate/octyl gallate分类
生物科学引用本文复制引用
丛蕾蕾,纪晓俊,聂志奎,彭超,黎志勇,邓中涛,黄和..花生四烯酸油脂高产菌株的选育[J].生物加工过程,2012,10(5):34-38,5.基金项目
国家重点基础研究发展计划(973计划)资助项目(2011CBA00802) （973计划）
国家自然科学基金资助项目(21006049) （21006049）
国家科技支撑计划资助项目(2011BAD23B03) （2011BAD23B03）
长江学者和创新团队发展计划资助项目(IRT1066) （IRT1066）
江苏高校优势学科建设工程资助项目 （）
