山西医科大学学报2012，Vol.43Issue(9)：655-657,723,4.DOI:10.3969/J.ISSN.1007-6611.2012.09.005
Ⅰ型胶原在丝素蛋白-羟基磷灰石类骨质复合生物材料修复兔股骨缺损中的表达
Type Ⅰ collagen expression after the rabbit defected bone repaired with silk fibroin-hydroxyapatite osteoid composite biomaterials
摘要
Abstract
Objective To evaluate the bone formation ability of silk fibroin-hydroxylapatite osteoid composite biomaterial in vivo. Methods The materials were implanted to the femoral medullary cavity of the New Zealand white rabbits with silk fibroin-hydroxyapatite osteoid composite biomaterials in experiment group and hydroxyapatite in control group. At weeks 4,8,12,specimens were removed. Type I collagen was stained by immunohistochemical staining and the integral optical density of type I collagen was measured by Motic Med 6.0 image anylysis system. Results Immunohistochemical staining showed that the positive staining of type I collagen in experi ment group was higher than that in control group respectively at weeks 4,8,12. The integral optical density of positive expression of type I collagen in experiment group was higher than that in control group at different time(P <0.05). Conclusion Bone formation ability of silk fibroin-hydroxyapatite osteoid composite biomaterials is better than that of pure hydroxyapatite. And it is expected to become a new type of bone substitute materials.关键词
Ⅰ型胶原/成骨性能/免疫组织化学染色Key words
type I collagen/bone formation ability/immunohistochemical staining分类
医药卫生引用本文复制引用
邹蕊,石福乔,牛林,白乐康..Ⅰ型胶原在丝素蛋白-羟基磷灰石类骨质复合生物材料修复兔股骨缺损中的表达[J].山西医科大学学报,2012,43(9):655-657,723,4.基金项目
陕西省科技攻关基金资助项目(2008 14K-03) （2008 14K-03）
