山西医科大学学报2012，Vol.43Issue(9)：713-716,4.DOI:10.3969/J.ISSN.1007-6611.2012.09.022
基于正交设计大鼠脑动脉血管分叉冠状冰冻切片制备方法研究
Improvement on preparation of coronal frozen section from rat cerebral artery bifurcation based on orthogonal design
伍刚 1许百男 2周敬安 1刘策 1刘广韬 1李双彦1
作者信息
- 1. 中国人民解放军第309医院神经外科,北京100091
- 2. 中国人民解放军总医院神经外科
- 折叠
摘要
Abstract
Objective To optimize the conditions for preparing the rat cerebral artery bifurcation coronal frozen sections. Methods Three-factor and three-level orthogonal design was processed with SPSS 13. 0. Three factors were frozen temperature, section thickness and quick-freezing time above liquid nitrogen. The data from orthogonal design mean square chart and single factor charts were collected and analyzed. Results The optimized conditions for rat cerebral artery bifurcation coronal frozen section were frozen temperature at — 25℃ , section thickness of 4 μm and quick-freezing time above liquid nitrogen of 15 s. Conclusion The optimized experiment factors could be quickly obtained for rat cerebral artery bifurcation coronal section by SPSS 13.0.关键词
血管分叉/正交设计/冰冻切片Key words
vessel bifurcation/orthogonal design/frozen section分类
医药卫生引用本文复制引用
伍刚,许百男,周敬安,刘策,刘广韬,李双彦..基于正交设计大鼠脑动脉血管分叉冠状冰冻切片制备方法研究[J].山西医科大学学报,2012,43(9):713-716,4.
