现代检验医学杂志2012，Vol.27Issue(4)：55-57,3.DOI:10.3969/j.issn.1671-7414.2012.04.021
HCV抗原检测与HCV-RNA和HCV抗体检测的比较研究
Comparative Study on the Detection of HCV-Antigen, HCV-RNA and HCV-Antibody Detection
李妙羡 1王香玲 1吴晓康 1卢洁 1王小利 1尹佳峰 1孟昊1
作者信息
- 1. 西安交通大学医学院第二附属医院检验科,西安710004
- 折叠
摘要
Abstract
Objective To study the value of measurement of HCV antigen, HCV antibody and HCV-RNA in the clinical diagnosis of Hepatitis. Methods HCV antigen would adopt double antibody sandwiched method; HCV-RNA would employ the real-time fluorescence quantitative PCR technique, HCV antibody would use the technology of Enzyme-Linked Immunosor-bent Assay (ELISA) ,44 specimens of positive HCV antibody would be detected with HCV antigen and HCV-RNA measurement. Results Among the specimens of positive HCV antibody, the rate of positive HCV antigen was measured as 43. 2%;the rate of positive HCV-RNA was measured as 82. 5%. Among the specimens of positive HCV-RNA,the rate of positive HCV antigen was measured as 45. 5%. Conclusion In the three kinds of Hepatitis markers,it was the most reliable method to judge whether infected with Hepatitis by using the real-time fluorescence quantitative PCR technique to measure HCV-RNA. If HCV antigen was used to diagnose Hepatitis, there was still 54. 5% rate of missed detection. Hence,there exists a long distance from the application in the clinical practice. In the hospitals where it was impossible to employ the realtime fluorescence quantitative PCR technique to measure HCV-RNA,when confirming the specimens of positive HCV antibody with HCV antigen and judging past exposure or present exposure to HCV,index of liver function and clinical manifestation should be combined to make a clear and definite diagnosis.关键词
丙肝/HCV抗原/HCV抗体/HCV-RNAKey words
hepatitis C/ HCV-antigen/ HCV-antibody/ HCV-RNA分类
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李妙羡,王香玲,吴晓康,卢洁,王小利,尹佳峰,孟昊..HCV抗原检测与HCV-RNA和HCV抗体检测的比较研究[J].现代检验医学杂志,2012,27(4):55-57,3.
