现代检验医学杂志2012，Vol.27Issue(5)：51-53,56,4.DOI:10.3969/j.issn.1671-7414.2012.05.017
连云港地区大肠埃希菌质粒介导喹诺酮类抗菌药耐药基因检测
Detection of Plasmid-mediated Quinolone Resistance Gene in Escherichia Coli from Lianyungang Location
张丽 1茆海丰 1王春梅1
作者信息
- 1. 连云港市第一人民医院检验科,江苏连云港,222002
- 折叠
摘要
Abstract
Objective To investigate the appearance of plasmid-mediated quinolone resistance genes in Enterobacter coli from Lianyungang location for clinical treatment. Methods Detection of plasmid-mediated resistance gene qnrA, qnrB, qnrS and aac (6')-Ib-cr of 88 clinical isolated Escherichia coli strains were performed by PCR. Gene-positive PCR products were se-quenced to confirm positive results,and by the aac (6')-Ib sequence alignment with Genbank registration to determine the aac (6')-Ib-cr. Analysis drug susceptibilty results of positive strains. Results In 88 clinical strains of E. Coli detected ,7 strains were detected in qnr-positive (7. 95%), including qnrB positive 2(2. 27%),qnrS positive 5(5. 68%), not detected qnrA positive strains. Detection aac (6')-Ib positive isolates 12 (13. 64%) ,of which 6 strains (6. 82%) were aac (6')-Ib-cr. Detected in 12 qnr and aac(6')-Ib-cr gene positive E. Coli, 8 strains of quinolone resistance, 3 strains sensitive to quinolones, one strain was levofloxacin sensitive but resistant tociprofloxacin,11 were ESBLs producting strains. Conclusion Evidences suggested the emergence of plasmid-mediated quinolone resistance gene-borne species in clinical isolated E. Coli in Linayungang location,and it often coexist with ESBLs,the monitoring and control should be strengthened in order to prevent the transmission and spreading of these strains.关键词
大肠埃希菌/喹诺酮类/qnr基因/aac(6')-Ib-cr基因/耐药机制/质粒介导Key words
Escherichia coli/quinolone/qnr gene/aac(6')-Ib-cr gene/resistance mechanisms/plasmid-mediated分类
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张丽,茆海丰,王春梅..连云港地区大肠埃希菌质粒介导喹诺酮类抗菌药耐药基因检测[J].现代检验医学杂志,2012,27(5):51-53,56,4.
