现代检验医学杂志2012，Vol.27Issue(5)：95-96,99,3.DOI:10.3969/j.issn.1671-7414.2012.05.031
石蜡包埋乳腺癌组织DNA提取方法
Paraffin-embedded Breast Cancer Tissue DNA Extraction Method
康毓芝 1王永兴 1杨宝珍 2韩恩善3
作者信息
- 1. 陕西省肿瘤医院检验科,西安,710061
- 2. 宁夏医科大学附属医院中心实验室,银川,750004
- 3. 宁夏医科大学附属医院病理科,银川,750004
- 折叠
摘要
Abstract
Objective Explore DNA extraction methods of the paraffin-embedded tissue in breast cancer patients and verified it. Methods 70 cases of large areas of bleeding in 10 ml/dl formalin fixed paraffin-embedded breast cancer tissue with avoid autolysis , degeneration, or organizational structure was unclear, and prevent cross-contamination. After TES solution water bathed dewaxing,successively with PBS buffer (pH7. 2) and 100 ml/dl,95 ml/dl,75 ml/dl ethanol concentration gradient of 60°C incubation of 4 hours,Phenol-chloroform extraction DNA. By polymerase chain reaction (PCR) ,agarose gel electropho-resis and ultraviolet spectrophotometry to measured the A260nm /A280nm value analysis of the quality of the extracted DNA. Results 62 cases (88.57%) paraffin tissue specimen DNA successfully extracted obtained DNA samples from the A260nm/ A280nm ratio (1. 78 + 0. 16) ,This condition of the sample availability of a sufficient number of complete DNA extraction rate, to meet the follow-up clinical research needs. Conclusions The method improved extracting DNA from paraffin-embedded simple,economic and it is ideal means to extract DNA from paraffin-embedded tissues,关键词
DNA提取/石蜡包埋组织/聚合酶链反应Key words
DNA extraction/formalin-fixed paraffin-embedded tissues/polymerase chain reaction分类
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康毓芝,王永兴,杨宝珍,韩恩善..石蜡包埋乳腺癌组织DNA提取方法[J].现代检验医学杂志,2012,27(5):95-96,99,3.
