现代检验医学杂志2012，Vol.27Issue(5)：100-101,104,3.DOI:10.3969/j.issn.1671-7414.2012.05.033
自制HLA-B27基因检测试剂盒的应用
Application of the Self-made Human Leukocyte Antigen HLA-B27 Test Kits
摘要
Abstract
Objective To verify the accuracy of the self-made human leukocyte antigen B27 (HLA-B27) detect kit. Methods According to the principle of sequence specific primer polymerase chain reaction (SSP-PCR), matched the HLA sequence on database of HLA,designed the B27 primer sequences to specific amplification of B27 fragment,and thus to optimize the PCR conditions and reaction system,self-made B27 test kit to detect B27 gene from EDTA anticoagulant blood of 254 cases of suspected ankylosing spondylitis,acute uveitis,the test results was compared with control kits,and analysed the consistency of them. Results No significant differences were found between two kind kits( χ2 = 0. 125 , P= 0. 727>0. 05). Conclusion A new simple,fast and low cost kit is recommended in clinical application in the future.关键词
人类白细胞抗原-B27/序列特异性引物聚合酶链反应/试剂盒/强直性脊柱炎Key words
HLA-B27 / SSP-PCR/ kits/ ankylosing spondylitis分类
医药卫生引用本文复制引用
郭华,张海祥,孙丽君,王海芳,赵院利,梁导艳,刘杨,胡军..自制HLA-B27基因检测试剂盒的应用[J].现代检验医学杂志,2012,27(5):100-101,104,3.基金项目
陕西省重大科技创新项目[2007ZKC(一)06-02] （一）
西安市科技计划项目(YF07189). （YF07189）
