实用妇产科杂志2012，Vol.28Issue(7)：565-570,6.
产妇子宫平滑肌组织中MAPK信号转导通路及CX43表达与产后出血关系的研究
Expression and Significance of Mitogen-Activated Protein Kinase Pathway and Connexin43 in Myometrium of Parturient Women with Postpartum Hemorrhage
摘要
Abstract
Objective:To investigate the contractility and contract potentiality of myometrium from parturient women with postpartum hemorrhage by uterine atony,and to determine the expression and significance of mitogen-activated protein kinase (MAPK) pathway and CX43 in the pathogenesis of postpartum hemorrhage ( PPH). Methods:30 parturient with PPH, who presented to Fujian Maternity and Child Health Hospital for regular prenatal care and parturition from November 2009 to December 2010, were selected as the study group. 30 health parturient at the same period were chosen as control. Isometric tension recording was used to detect the contractility and its potentiality induced by oxytocin. The levels of CX43, phosphorylation of ERKi/2,JNK1/2 and p38MAPK in myometrium of study group and control were determined by western blotting: The mRNA of CX43 was detected by Real-time fluorescent quantitative reverse transcription PCR. Results : (1)The spontaneous contractility and frequency of myometriums in the study group were significanctly lower than those of control(P＜0.05). The frequency,range ,contractility and potentiality of the constriction induced by oxytocin in study group were lower than those of control( P＜0.05, P＜0.01). (2) When contractility of 72.66 g · times per hour was chosen as diagnostic point of postpartum hemorrhage by uterine atony , the area under curve of ROC was 0.802. (3)In the study group,the expressions of p-ERK1/2,p-JNK1/2, p-P38,CX43mRNA and their protein levels in myometrium were significantly lower than those of control (P＜ 0.05). (4)There were positive correlations between the expressions of p-ERK1/2, p-JNK1/2, p-P38, CX43mRNA and the protein levels in myometrium and the contraction activity in both groups(P＜0.05). (5) The CX43mRNA levels and its protein quantum were siginificantly correlated with the expression of p-ERK1/ 2,p-JNK1/2 and p-P38 in uterine smooth muscle in both groups( P＜0. 05). Conclusions:The contractility and contract potentiality of myometrium in PPH women were much lower than those of control. The expressions of MAPKs and CX43 in myometrium of PPH women were also much lower than those of control,and they are closely correlated. So the interruption of MAPK signal pathway and the decreased exprssion of CX43 in myometrium may be involved in the pathogenesis and development of PPH.关键词
产后出血/宫缩乏力/丝裂原激活蛋白激酶信号通路/连接蛋白43Key words
Postpartum hemorrhage/Uterine atony/ Mitogen-activated protein kinase pathway/Connexin43分类
医药卫生引用本文复制引用
颜建英,黄科华,刘青闽,黄晓燕,徐榕莉,余爱丽,邓婷..产妇子宫平滑肌组织中MAPK信号转导通路及CX43表达与产后出血关系的研究[J].实用妇产科杂志,2012,28(7):565-570,6.基金项目
福建省科技计划重点项目(编号:2009Y0008) （编号:2009Y0008）
福建省医学创新课题(编号:2009-CXB-32) （编号:2009-CXB-32）
