实用医学杂志2012，Vol.28Issue(12)：1946-1948,3.DOI:10.3969/j.issn.1006-5725.2012.12.007
过表达Src诱导K562细胞产生格列卫耐药
Over-expression of Src kinase inducing imatinib resistance of K562 cells
摘要
Abstract
Objective To explore the effects of over-expression of Src kinase on drug-resistance of K562 cells. Methods Gene transfection was conducted to over-express Src kinase in K562 cells, and the expression levels were tested by Western blot. The anti-proliferate effects of imatinib on these transfected cells were tested by WST assay. Then the expression of Src kinase was suppressed by siRNA, and the proliferation of imatinib resistant K562/G cells was observed. Results Over-expression of Src kinase could induce imatinib resistance in K562 cells. Inhibiting Src expression with Src siRNA for 24h could inhibit almost 47% of K562/G cells proliferation. Conclusion Src kinase plays a pivotal role in the induction of imatinib-resistance of K562 cells. Src kinase may be an attractive target for treating imatinib resistant chronic myeloid leukemia.关键词
Src族激酶类/K562细胞/格列卫耐药Key words
Src family kinase/ K562 cells/ Imatinib resistance引用本文复制引用
嘉红云,周强,李娟,叶润清,邓小燕,吴晓蔓..过表达Src诱导K562细胞产生格列卫耐药[J].实用医学杂志,2012,28(12):1946-1948,3.基金项目
国家自然科学基金(编号:81101317) （编号:81101317）
广州市医药卫生科技项目(编号:201102A213018) （编号:201102A213018）
广州医学院博士启动基金(编号:2009-8) （编号:2009-8）
