生殖医学杂志2012，Vol.21Issue(5)：465-471,7.DOI:10.3969/j.issn.1004-3845.2012.05.012
前动力蛋白2/前动力蛋白受体2基因杂合突变小鼠表型特征
Phenotypes of PK2/PKR2 heterozygosis mutant mice
肖玲 1周闻白 2周群勇 3胡仁明2
作者信息
- 1. 复旦大学附属金山医院内分泌科,上海200540
- 2. 复旦大学附属华山医院内分泌科,上海 200040
- 3. 美国加州大学欧文分校医学院药理部,美国加州 92697
- 折叠
摘要
Abstract
Objective; To observe the phenotype changes including weight growth and reproductive system development in PK2/PKR2 heterozygosis mutant mice.
Methods; The weight gain and external genitalia development were compared between the three heterozygosis mutant mice groups and the wild type group. GnRH neurons distribution was observed by fluorescent staining method in four groups. The stage of estrus cycle was determined by cytologic evaluation of vaginal smears. The vaginal exfoliate cells were smeared on glass slides, and the cytologic features were evaluated under microscopy
Results; There were no changes in weight growth, external genitalia development and the GnRH neurons distribution in brain between the three mutant mice groups and the wild type mice group. The length of estrus cycle was extended in heterogeneous mice, especially in PK2/PKR2 heterogeneous mutation mice.
Conclusions: Loss of one copy of PK2 or PKR2 gene did not change reproductive system and GnRH neurons development except estrous cycle. So PK2/PKR2 pathway may play roles in the regulation of reproductive system function.
关键词
前动力蛋白2/前动力蛋白受体2/杂合突变/外生殖器/GnRH神经元/动情周期Key words
Prokineticin 2/ Prokineticin receptor 2/ Heterozygosis mutation/ External genitalia/ GnRH neurons/ Estrous cycle引用本文复制引用
肖玲,周闻白,周群勇,胡仁明..前动力蛋白2/前动力蛋白受体2基因杂合突变小鼠表型特征[J].生殖医学杂志,2012,21(5):465-471,7.
