天然产物研究与开发2012，Vol.24Issue(8)：1089-1093,5.
酶解大豆蛋白产天冬氨酸蛋白酶抑制剂的研究
The Research of Aspartic Protease Inhibitors from Soy Protein Hydrolyzed by Proteases
张国强 1田亚平1
作者信息
- 1. 江南大学工业生物技术教育部重点实验室,无锡214122
- 折叠
摘要
Abstract
In this paper, we reported a kind of aspartie protease inhibitor from soy protein ( soy aspartie proteinase inhibitor,SAPI) after controlled enzymatic hydrolysis. The final inhibitory activity was 254.2 IU /mg and the purification fold was 62 after DEAE-52 ion-exchange chromatography,Superdex Peptide 10/300 GL gel chromatography and SOURCE 15RPC reversed-phase chromatography. The IC50, value of SAPI was 25. 67 μg/mL to pepsin(3000 U) , SAPI showed good thermostability and belonged to a kind of non-competitive inhibitor.
关键词
酶解/大豆蛋白/天冬氨酸蛋白酶/抑制剂Key words
enzymolysis/ soy protein/ aspartie proteinase/ inhibitor分类
生物科学引用本文复制引用
张国强,田亚平..酶解大豆蛋白产天冬氨酸蛋白酶抑制剂的研究[J].天然产物研究与开发,2012,24(8):1089-1093,5.
