天然产物研究与开发2012，Vol.24Issue(8)：1098-1104,7.
基于响应面法从连翘叶中提取连翘酯苷A和连翘苷的工艺优化
Optimization of Extraction Technology of Forsythiaside and Phillyrin in Forsythia suspense Leaves Based on Response Surface Methodology
摘要
Abstract
Response surface methodology (RSM) was applied to optimize the extraction technology of forsythiaside and phillyrin from Forsythia suspense leaves. Base on single factor experiment, the influence factors of liquid to solid ration, ethanol concentration, extraction time and extraction temperature were studied on the yields of forsythiaside and phillyrin. According to Box-Behnken combination method (Design expert 8. 0. 5 b) ,the optimal conditions were determined as follows:ratio of liquid to solid 30 mL/g,ethanol concentration 53% .extraction time 53 min,and extraction temperature 74 t. Under the conditions, the yields of forsythiaside and phillyrin were 7.56% and 3.09% ,respectively, and the relative error between the testing values (7. 53% and 3. 03% .respectively) and theoretic values were 0. 4% and 2. 0% .respectively.关键词
连翘叶/连翘酯苷A/连翘苷/响应面法/HPLCKey words
Forsythia suspense leaves/response surface methodology/forsythiaside/phillyrin/HPLC分类
医药卫生引用本文复制引用
刘伟霞,杨建雄..基于响应面法从连翘叶中提取连翘酯苷A和连翘苷的工艺优化[J].天然产物研究与开发,2012,24(8):1098-1104,7.基金项目
陕西省科技攻关项目(2004K18-G10) （2004K18-G10）
