天然产物研究与开发2012，Vol.24Issue(9)：1182-1185,1195,5.
丹参酮ⅡA对正常和氧化损伤内皮细胞的保护作用研究
Protective Effect of Tanshinone ⅡA on Normal and Oxidative Injured Endothelial Cells
摘要
Abstract
The oxidative injury model established with H2O2 was used to study the protective effects of Tanshinone IIA on the oxidative damage of HUVECs. The activities of LDH, SOD, NOS and GSH-Px, and the contents of NO and MDA were determined in each experimental groups. The livability of HUVECs was determined by MTT assay and flow cytometry was employed to analysis the influences on the normal and the oxidative injury HUVECs. The results showed that Tanshinone IIA has no cytotoxicity at 10-100 ujnoI/L of concentration. The ability of protecting HUVECs from oxidative injury was enhanced obviously at more than 20 ujnol/L of Tanshinone IIA and the releasing percentage of LDH and the content of MDA were decreased while the activity of NOS, NO, SOD and GSH-PX were increased. The apoptosis and the rate of the death were reduced significantly. Results indicated that Tanshinone IIA could prevent HUVECs from oxidative damage which caused the formation of atherosclerotic plaques.关键词
丹参酮ⅡA/氧化损伤/人脐静脉内皮细胞/保护作用Key words
Tanshinone IIA/oxidative damage/HUVECs/protective effect分类
医药卫生引用本文复制引用
罗婷,钟先锋,李静,刘小如,邓泽元..丹参酮ⅡA对正常和氧化损伤内皮细胞的保护作用研究[J].天然产物研究与开发,2012,24(9):1182-1185,1195,5.基金项目
食品科学与技术国家重点实验室(南昌大学)自由探索课题(SKLF-TS-200921) （南昌大学）
2011年江西省科技厅对外合作计划 （）
