天然产物研究与开发2012，Vol.24Issue(9)：1284-1287,4.
南瓜叶中γ-氨基丁酸的提取及薄层扫描测定
Determination and Extraction of γ-Aminobutyric Acid from Pumpkin (Cucurbita mosehata) Leaves
摘要
Abstract
The extraction conditions and thin layer chromatography scanning (TLCS) analytic method of y-aminobutyric acid from pumpkin leaves were studied. On the basis of single factor experiments, orthogonal test was investigated. The optimum parameters for extracting y-aminobutyric acid from pumpkin leaves were determined as follows;the concentration of alcohol was 20% , the ratio of solid to liquid was 1:17 ( w/v) ,and the extraction time was 1 h. Under these conditions,the yield of γ-aminobutyric acid was 209 mg/100 g. The calibration curve showed good linearity in the range of 0.125 to 2.0mg/mL (R2=0.9991) with the average recovery (n=4) of 99.61%.关键词
南瓜叶/γ-氨基丁酸/提取/含量测定Key words
pumpkin (Cucurbita mosehata) leaves/γ-aminobutyric acid/extraction/determination分类
农业科技引用本文复制引用
黄美娥,彭胜,陈阳波,袁洪昆..南瓜叶中γ-氨基丁酸的提取及薄层扫描测定[J].天然产物研究与开发,2012,24(9):1284-1287,4.基金项目
湖南省科学技术厅项目(2011TP4014-3) （2011TP4014-3）
湖南省教育厅项目(11C1043) （11C1043）
林产化工工程湖南省重点实验室开放基金项目(JDZ201004) （JDZ201004）
湖南省高校科技创新团队支持计划资助"林产资源化学林化产品研发"(林产化工工程湖南重点实验室) （林产化工工程湖南重点实验室）
