天然产物研究与开发2012，Vol.24Issue(9)：1297-1302,6.
大孔吸附树脂纯化海红果黄酮工艺的研究
Purification Technology of Malus micromalus Makino Flavonoids by Macroporous Absorbent Resins
摘要
Abstract
The suitable resin was selected by static and dynamic adsorption from 10 kinds of macroporous absorbent resins to Malus micromalus Makino flavonoids. The purification technology of the crude extracts by macroporous adsorbing resins were decided through the experiments. Hie results showed that NKA-9 resin was selected because it gave the most effective of separation. The optimum process for the purification was as follows: the concentration of sample solution was 5.15 mg/mL,pH was 4.0,the loading weight was 4 BV and the adsorption flow rate was 2 BV/h;then eluted with 80% ethanol for 3 BV with elution flow rate of 1 BV/h. In these optimum conditions,average adsorption rate.desorption rate, yield and purity of the flavonoids was reached to (79. 39 ± 0. 13) % , (84. 14 ± 0. 11) % , (68. 20 ± 0.15 ) % and (28.81 ±0.06)% (n = 5),respectively.关键词
海红果/黄酮/大孔吸附树脂/纯化Key words
fruit of Malus micromalus Makino/flavonoids/macroporous adsorption resin/purification分类
医药卫生引用本文复制引用
徐玉霞,王华斌..大孔吸附树脂纯化海红果黄酮工艺的研究[J].天然产物研究与开发,2012,24(9):1297-1302,6.基金项目
国家自然科学基金项目(41071359) （41071359）
宝鸡文理学院重点项目(ZK11021) （ZK11021）
陕西省重点学科自然地理学资助 （）
