天然产物研究与开发2012，Vol.24Issue(10)：1408-1411,4.
狭苞橐吾中几种倍半萜类化合物的活性筛选
Activity Screening on Sesquiterpene Compounds from Ligularia intermedia Nakai of Shanxi
摘要
Abstract
To study the biological activity of sesquiterpene compounds from Ligularia intermedia Nakai of Shanxi. With different concentrations of four kinds of sesquiterpenes intervening AH109 bacteria solution which carries P53 and SV40 tumor virus large T-Antigen genes,we adopted yeast two-hybrid system method for determination of beta-galactose gluco-side enzyme activity to analyze the interaction effects on P53 protein and SV40 large T-antigen by the four compounds. The results indicated that 8β-methoxyeremophil-7 (11 )-ene-12,8α(4/3,6α)-diolide (1) and 8β-hydroxyl-6β-angloxyer-emophil-7(ll)-ene-12,8α-olide-15-oic acid (2) had no significant effects on the interaction between P53 protein and SV40-large T-antigen. However,medium and high concentrations of furanoeremophilan-4β,6α-olide (3 ), low, medium and high concentration of 8β-hydroxyeremophil-7 (11 )-ene-12,8α(4/3,6α)-diolide (4) had an inhibitory effects on the interaction between P53 protein and SV40 large T-antigen. It showed that compounds 3 and 4 which extracted from Ligularia intermedia Nakai of Shanxi had biological inhibitory activity effects on the interaction between P53 and SV40-large T-Antigen of the yeast two-hybrid system.关键词
狭苞橐吾/倍半萜/活性筛选Key words
Ligularia intermedia/sesquiterpenes/activity screening分类
医药卫生引用本文复制引用
柴智,樊慧杰,王永辉,薛慧清..狭苞橐吾中几种倍半萜类化合物的活性筛选[J].天然产物研究与开发,2012,24(10):1408-1411,4.基金项目
山西省科技攻关项目(20080311052-1) （20080311052-1）
山西省自然科学基金资助项目(2009011013-1) （2009011013-1）
