天然产物研究与开发2012，Vol.24Issue(10)：1437-1440,1392,5.
人肠道菌对黄芩苷的生物转化
Biotransformation of Bacicalin by Human Intestinal Bacteria
摘要
Abstract
The characteristic of human intestinal bacteria biotransformation system was studied. The enzyme (β-D-glucu-ronidase) was isolated by Alcohol precipitation,and the factors on enzymatic properties were analyzed. The product was identified as baicalein by high performance liquid chromatography ( HPLC). After ultrasonic treatment on cultivation medium, the baicalein could be extracted. At pH 6.0 and 55 ℃ ,the activity of β-D-glucurom'dase reached maximum,and was activated by Ca2+ 、Mg2 ＋ and Cu2 + , but inhibited by Fe2 ＋ ; at 1 mmol/L Zn2 ＋ played a catalytic role, but an inhibitory factor at 5 mmol/L.关键词
肠道菌/生物转化/黄芩苷/超声波处理/β-D-葡萄糖醛酸苷酶Key words
intestinal bacteria/biotransformation/baicalin/ultrasonic treatment/β-D-glucuronidase分类
化学化工引用本文复制引用
刘伶文,司磊,任树勇,刘永红..人肠道菌对黄芩苷的生物转化[J].天然产物研究与开发,2012,24(10):1437-1440,1392,5.基金项目
陕西省教育厅专项科研项目(09JK453) （09JK453）
陕西省科技厅工业攻关项目(2008k07-14) （2008k07-14）
