天然产物研究与开发2012，Vol.24Issue(10)：1454-1457,1479,5.
超声辅助提取凤丹籽总黄酮的工艺研究
Ultrasonic-assisted Extraction Technology of Total Flavonoids from Paeonia ostii T.Hong et J.X.Zhang seed
摘要
Abstract
Extraction conditions of total flavonoids from Paeonia ostii T. Hong et J. X. Zhang seed with ethanol combining ultrasonic were studied. Through single factor and Box-Behnken response surface analytical method,the scope and combination of influencing factor on extraction yield of total flavonoids were confirmed. The response surface methodology was employed to evaluate the effects of alcohol concentration,solid to liquid ratio,ultrasonic extraction time,ultrasonic power and their interaction. Results showed that extraction time on the extraction yield of total flavonoids had a significant effect. The optimum conditions of flavonoid extraction were as follows; alcohol concentration 75% , Solid to liquid ratio 1:21 g/mL, ultrasonic extraction time 49 min and ultrasonic power 240 w,in this condition,the extraction yield of total flavonoids was 9.015 mg/g.关键词
凤丹籽/总黄酮/紫外分光光度法/超声提取/Box-Behnken响应面设计Key words
Paeonia ostii T. Hong et J. X. Zhang seed/total flavonoids/ UV-spectrophotometry/ultrasonic-assisted/ Box-Behnken response surface analytical method分类
农业科技引用本文复制引用
吴悠,李梅青,孙强,陈健,吴计划,王芬..超声辅助提取凤丹籽总黄酮的工艺研究[J].天然产物研究与开发,2012,24(10):1454-1457,1479,5.基金项目
铜陵市科技局项目(2010GY14) （2010GY14）
