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响应面法优化胡芦巴种子多糖提取工艺

何彦峰 杨仁明 胡娜 索有瑞 王洪伦

天然产物研究与开发2012,Vol.24Issue(10):1463-1467,5.
天然产物研究与开发2012,Vol.24Issue(10):1463-1467,5.

响应面法优化胡芦巴种子多糖提取工艺

Optimization of Extraction Process of Polysaccharides from Fenugreek (Trigonella foenum-graecum L.) by Response Surface Methodology

何彦峰 1杨仁明 2胡娜 1索有瑞 2王洪伦1

作者信息

  • 1. 中国科学院西北高原生物研究所,西宁810001
  • 2. 中国科学院研究生院,北京100049
  • 折叠

摘要

Abstract

Single-factor tests and response surface method (RSM) were used to optimize the extraction process of fenugreek seed polysaccharides (FSP). In order to obtaining the maximum of extraction yield of the polysaccharides, three main factors including extraction temperature, extraction times and ratio of raw material to water were selected with the Box-Behnken design and a quadric regression equation for predicting the yield of polysaccharides was established. The results showed that the optimum extraction conditions were as follows:extraction temperature 85 ℃ .extraction times 1.2 h,and ratio of raw material to water 1- 28. Under the conditions, the optimum extracting yield of FSP was 19. 89% , which is well matched with the predictive extracting yield 20.24%.

关键词

胡芦巴/多糖/响应面法/提取工艺

Key words

fenugreek/polysaccharides/response surface methodology/extraction process

分类

轻工纺织

引用本文复制引用

何彦峰,杨仁明,胡娜,索有瑞,王洪伦..响应面法优化胡芦巴种子多糖提取工艺[J].天然产物研究与开发,2012,24(10):1463-1467,5.

基金项目

中国科学院生命科学领域优秀青年科技专项(KSCX2-EW-Q-23) (KSCX2-EW-Q-23)

天然产物研究与开发

OA北大核心CSCDCSTPCD

1001-6880

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