太原理工大学学报2012，Vol.43Issue(4)：435-439,5.
异优呫吨酮及其衍生物与DNA相互作用的研究
Spectrophotometric Study on the Interaction of Isoeuxanthone and Piperidinyl Isoeuxanthone Derivative with DNA
摘要
Abstract
The binding mode and affinity of isoeuxanthone (1 , 6-dihydroxyxanthone) (A) and it's piperidinyl derivative (l-Hydroxy-6-(2-(l-piperidinyl) ethoxy) xanthone) (B) with calf thy-mus DNA were studied using absorption spectroscopy, fluorescence spectroscopy, circular di-chroism spectroscopy (CD) and fourier transform infrared (FTIR) difference spectroscopy. It was found that the two compounds showed apparent hypochromism with increasing concentrations of DNA, The binding constants Kb for A and B were found to be 1. 5 × 104 L/mol and 2. 8× 104 L/mol, respectively. The fluorescent intensity of DNA-EB system was quenched upon addition of A and B. The results suggest that the compounds interacted with DNA by intercalation mode and that the binding affinity of B was higher than that of A according to the calculated binding constants and fluorescent quenching constants. Moreover, the partial unwinding of the helix and the stabilization of base pairs were reflected in the changes of CD spectra. The characteristic absorption bands of FTIR attributed to base pairs also shifted. The spectral changes of intensity and shift of the B-DNA indicated more evident conformation change on addition of B than A, which is in agreement with the result of absorption spectroscopy and fluorescence spectroscopy.关键词
异优呫吨酮/1-羟基-6-(2-(1-哌啶基)乙氧基)呫吨酮/DNA/插入方式Key words
isoeuxanthone/ l-Hydroxy-6-(2-(l-piperidinyl)ethoxy)xanthone/ DNA/ intercalation mode分类
化学化工引用本文复制引用
王慧芳,魏丽乔,晏泓,高向华,许并社,唐宁..异优呫吨酮及其衍生物与DNA相互作用的研究[J].太原理工大学学报,2012,43(4):435-439,5.基金项目
山西省自然科学基金资助项目(2011011022-2) （2011011022-2）
山西省青年科技研究基金资助项目(2012021008-4) （2012021008-4）
