武警医学2012，Vol.23Issue(10)：859-861,865,4.
毛细管电泳法测定罗布麻叶中芦丁、异槲皮苷和槲皮素的含量
Determination of three flavonoids in Apocynum venetum by high-performance capillary electrophoresis
李芳 1马晓丽 2孙莲 3孟磊2
作者信息
- 1. 830063,乌鲁木齐,武警兵团指挥部医院药剂科
- 2. 830011,乌鲁木齐,新疆医科大学分析测试中心
- 3. 830011,乌鲁木齐,新疆医科大学药学院
- 折叠
摘要
Abstract
Objective To establish a high -performance capillary electrophoretic (HPCE) method for simultaneous determination of contents of rutin, isoquercitrin, and quercetin in Apocynum venetum. Methods A bare fused — silica capillary (58.5 cm x 75 (j,m, effective length of 48. 5 cm) was used. The buffer was composed of 50 mmol/L Na2B4O7 ( pH 9. 60) . The running voltage was set at 20 kV with polarity from anode ( inlet) to cathode ( outlet) . The separation time was 20 min and temperature was set at 25 'C . Online detection was performed at 350 nm ( PDA). Results The three types of flavonoids were well separated in 25 minutes and linear relationship in the peak area was good. The linear range was 4.4—44, 2.8—28.2, and3.8—38 (j,g/ml, respectively. The correlation coefficient was 0. 9968, 0. 9995 , and 0. 9991, respectively. The average recovery was 97. 3% , 98. 4% , and 98. 3% , respectively. Conclusions This method is simple, rapid, accurate, and reliable, which can be used for content determination of flavonoids in Apocynum leaves.关键词
罗布麻叶/芦丁/异槲皮苷/槲皮素/黄酮/毛细管电泳法Key words
Apocynum leaves/ rutin/ quercitrin/ quercetin/ flavonoids/ capillary zone electrophoresis
分类
医药卫生引用本文复制引用
李芳,马晓丽,孙莲,孟磊..毛细管电泳法测定罗布麻叶中芦丁、异槲皮苷和槲皮素的含量[J].武警医学,2012,23(10):859-861,865,4.
