西安交通大学学报（医学版）2012，Vol.33Issue(6)：723-727,5.
AMP-18在胆汁对胃黏膜细胞损伤中的作用
Effects of bile on AMP-18 expression in normal human gastric mucosal epithelial cell injury
摘要
Abstract
Objective To explore the effects of AMP-18 on gastric mucosa from rat model and cultured eternalized human gastric mucosa epithelium GES-1 cells. Methods Totally 44 healthy SD rats, half males and half females, were divided into 4 groups: duodenogastric reflux (DGR) group, bile duct ligation (BDL) group, DGR plus BDL group, and control group. Rats were killed 12 weeks after the operations. Cultured eternalized human gastric mucosa epithelium GES-1 cells were treated with media containing 6 different kinds of bile salts and 3 different kinds of bile acids and their mixture with different concentrations: glycochenodeoxycholate (GCDC), glycodeoxycholate (GDC), glycocholate (GO, taurochenodeoxycholate (TCDC), taurodeoxycholate ( TDC ), taurocholate (TO, lithocholic acid (LCA), cholic acid (CA), deoxycholic acid (DCA) (500 μmol/L, 1 000 μmol/L), mixture of bile salts (DY), and mixture of bile acids (DS) (1 500 μmol/L, 2 000 μmol/L). Rats in the control group were treated with normal media without bile salts or bile acids. RT-PCR was used to examine the expression of AMP-18 mRNA in rat gastric mucosa and human gastric mucosa epithelium. Results The expression of AMP-18 mRNA in the DGR group decreased significantly compared with that in the DGR plus BDL group, BDL group and control group (P<0. 05). The expression of AMP-18 mRNA in DGR plus BDL and BDL groups decreased compared with that in the control group, but there was no significant difference (P>0.05). The expression of AMP-18 mRNA was lower in the GC group than in the control group, without significant difference (P>0. 05). The expression of AMP-18 mRNA in the other groups was significantly lower than that in the control group (P< 0.05) in a dose-dependent manner. Conclusion AMP-18 plays an important role in the restoration of gastric mucosal epithelial cell injury.关键词
胆汁反流/胃黏膜上皮细胞/AMP-18Key words
bile reflux/gastric mucosal epithelial cell/AMP-18分类
医药卫生引用本文复制引用
宋银雪,龚均..AMP-18在胆汁对胃黏膜细胞损伤中的作用[J].西安交通大学学报（医学版）,2012,33(6):723-727,5.基金项目
卫生部临床学科重点项目基金资助(No.20012130) （No.20012130）
