西安交通大学学报（医学版）2012，Vol.33Issue(6)：760-764,5.
靶向沉默Notch1基因对胃癌细胞SGC-7901增殖和侵袭的影响
Effect of silencing Notch1 on proliferation and invasion of gastric cancer cell line SGC-7901
魏光兵 1常远鸿 2王康 1仇广林 1柴明明1
作者信息
- 1. 西安交通大学医学院第一附属医院普通外科,陕西西安710061
- 2. 西安第四医院消化内科,陕西西安710004
- 折叠
摘要
Abstract
Objective To investigate the effect of silencing Notchl gene on cell proliferation in vitro in gastric cancer cell line SGC-7901 and the underlying mechanisms. Methods The siRNA of notchl was transfected into gastric cancer cell line SGC-7901 by Lipofectamine 2000. Proliferation and invasion of SGC-7901 were detected after transfection by methabenzthiazuron assay and transwell assay, respectively. The mRNA expression of Notchl was determined by RT-PCR. The protein expressions of Cyclin Dl, Cyclin Al and CDK2 were determined by Western blotting. The mRNA expressions of MMP-2 and COX-2 were determined by RT-PCR. Results Compared with that in control group, the mRNA expression of Notchl was significantly decreased after transfection. The proliferation and invasion of SGC-7901 cells were inhibited significantly after Notchl silencing. Additionally, the protein expressions of Cyclin Dl and Cyclin Al as well as the mRNA expressions of MMP-2 and COX-2 were obviously inhibited. However, silence targeting Notchl did not affect CDK2. Conclusion RNA silence targeting Notchl can inhibit the proliferation and invasion of gastric cell line SGC-7901 probably through decreasing the expressions of Cyclin Dl and Cyclin Al and down-regulating the expressions of MMP-2 and COX-2, respectively.关键词
胃癌/肿瘤增殖/肿瘤侵袭/Notch1/RNA干扰Key words
gastric cancer/tumor proliferation/tumor invasion/Notchl/RNA interference分类
医药卫生引用本文复制引用
魏光兵,常远鸿,王康,仇广林,柴明明..靶向沉默Notch1基因对胃癌细胞SGC-7901增殖和侵袭的影响[J].西安交通大学学报（医学版）,2012,33(6):760-764,5.
