西安交通大学学报（医学版）2012，Vol.33Issue(6)：790-792,796,4.
流动注射化学发光法测定氢溴酸加兰他敏和力可拉敏
Flow-injection chemiluminescence method for the determination of lycoramine and galanthamine
宋翠翠 1唐玉海 1史喜燕 1张广彬 1李明月 1李昕沛1
作者信息
- 1. 西安交通大学理学院分析科学研究所,陕西西安710061
- 折叠
摘要
Abstract
Objective To develop a simple and rapid flow-injection chemiluminescence (FI-CL) method for the determination of galanthamine and lycoramine. Methods In an alkaline medium, galanthamine and lycoramine can obviously inhibit the chemiluminescence intensity of the reaction of luminol- K3Fe(CN)6 system, and the relative CL intensity is proportional to the concentration of galanthamine and lycoramine. Based on this, a new FI-CL method was developed to determine galanthamine and lycoramine. And the effects of chemical and instrumental variables were optimized. Results Under the optimized conditions, the relative CL intensity was proportional to the concentration within the range of 5×10-3 -10 mg/L for galanthamine and within the range of 5 ×10-2-10 mg/L for lycoramine. The detection limit was 0. 53 μg/L for galanthamine and 2. 60 μg/L for lycoramine, respectively. Conclusion The flow-injection chemiluminescence method can be applied to determine the content of galanthamine and lycoramine in pharmaceutical preparations and in serum.关键词
化学发光/鲁米诺/加兰他敏/力可拉敏/药物分析Key words
chemiluminescence/luminol/lycoramine/galanthamine/pharmaceutical analysis分类
社会科学引用本文复制引用
宋翠翠,唐玉海,史喜燕,张广彬,李明月,李昕沛..流动注射化学发光法测定氢溴酸加兰他敏和力可拉敏[J].西安交通大学学报（医学版）,2012,33(6):790-792,796,4.
