西北林学院学报2012，Vol.27Issue(6)：66-69,92,5.DOI:10.3969/j.issn.1001-7461.2012.06.13
栓皮栎AFLP反应体系的建立
Establishment of AFLP Reaction System in Quercus variabilis
摘要
Abstract
The objective of the paper was to establish an AFLP reaction system fitting for Quercus variabilis , after systematical study on several crucial aspects involving the process and time of the endonuclease digestion-ligation reaction, the preamplification and selective amplification. The results indicated that the extraction of the genomic DNA should choose the improved CTAB method from the tender leaves of Q. variabilis. One-step method, 200 ng DNA dosage and digestion and ligation 4 hours were the best combination for the endonuclease digestion-ligation reaction system The preamplification reaction was carried out using 20 diluted digestion and ligation products. And the selective amplification was conducted with no diluted preamplification products immediately. The effectiveness test in each step and primer screening test demonstrated that the system was suitable for the AFLP analysis of Q. variabilis.关键词
栓皮栎/AFLP/反应体系Key words
Quercus variabilis / AFLP/ reaction system分类
农业科技引用本文复制引用
杨阳,张存旭,郝丽丽,张文辉..栓皮栎AFLP反应体系的建立[J].西北林学院学报,2012,27(6):66-69,92,5.基金项目
林业公益性行业专项(21004011) （21004011）
西北农林科技大学唐仲英育种基金. （）
