现代科学仪器Issue(5)：133-135,3.
一种快速计算机集成成像
Determination of Environmental Hormone in Water by Yeast with h-ER Gene
摘要
Abstract
Objective:The concentration of enviromental hormone in the water accurately is determinated.Methods: After enviromental hormone is combined by the yeast with h-ER gene uniquely,β-galactosidase is produced. The concen- tration of enviromental hormone can be discovered by determinating the activity of the β-galactosidase. Result: The con- centration of 17[3-estradiol is related to the activity of the β-galactosidase in 'S' curve, when its concentration is between 10H to 109mol/L .(correlation coefficient is 0.984) .To make the determination more accurately ,the concentration should between 5.88 10-nmol/L to 1.44 10 μ mol/L.And then the addition standard recovery will between 74% to 108%. Conclu- sion: This method is accurate, reliable and with good repeatability in the recommendatory rang.It can be used in determi- nating the concentration of enviromental hormone in water and waste water.关键词
计算机集成成像/集成成像一次记录法/Direct3DKey words
Enviromental hormone/h-ER gene/Yeast分类
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赵洋甬,徐运,潘双叶,赵建平,沈碧君..一种快速计算机集成成像[J].现代科学仪器,2012,(5):133-135,3.基金项目
水污染源应急监测技术体系研究 （）
