畜牧兽医学报2012，Vol.43Issue(9)：1353-1359,7.
广西巴马小型猪白细胞抗原经典Ⅱ类基因克隆及生物信息学分析
Cloning and Bioinformatics Analysis of Swine Leukocyte Antigens Class Ⅱ Genes in Guangxi Bama Minipigs
摘要
Abstract
The study aimed to elucidate the structures of SLA Class ? genes of Guangxi Bama Minipigs and the potential in pigs to humans xenotransplantation. 4 specimens of ear tissues were sampled from four Guangxi Bama Minipigs, and the classical swine leukocyte antigens class ? genes (SLA-DRA, SLA-DRB1, SLA-DQA , SLA-DQB1) were amplified by PCR after reverse transcription. Purified products were cloned into pMD18-T vectors and bi-directional sequenced. Analyzing obtained sequences by BLAST in NCBI, a total of eight alleles were found, and five alleles have been found in previous reports, the other three were novel. From the homology analysis between alleles of SLA and HLA in GenBank and phylogenetic trees analysis, the results showed that the Guangxi Bama Minipigs possessed high homologous in MHC information with human, and indicated that Guangxi Bama minipigs would have potential utilization values in the study of pig to human xenotransplantation.关键词
主要组织相容性复合体/猪白细胞抗原/异种移植/小型猪Key words
MHC/ SLA/ xenotransplantation/ minipig分类
农业科技引用本文复制引用
刘子展,王在贵,李奎,郭亚芬,王爱德,张玉琼,杨述林..广西巴马小型猪白细胞抗原经典Ⅱ类基因克隆及生物信息学分析[J].畜牧兽医学报,2012,43(9):1353-1359,7.基金项目
国家科技支撑计划(2012BAI39B04 （）
2011BAI15B02) （）
"973"计划(2011CBA01005) （2011CBA01005）
"863"计划(SQ2011AAJY2795) （SQ2011AAJY2795）
