畜牧兽医学报2012，Vol.43Issue(9)：1369-1376,8.
PPARα和PPARγ基因在不同脂尾型绵羊脂肪组织中的发育性表达研究
Developmental Expression of PPARα and PPARγ mRNA in Adipose Tissues of Different Fat-tailed Sheep
摘要
Abstract
The objectives of this study were to investigate the developmental expression characteristics of PPARa and PPARy mRNA in two sheep breeds of distinct tail types and to discuss the probable relationship between the mRNA expression and metabolism of adipose tissue. Guangling Large Tailed (GLT) sheep and Small Tailed Han sheep (STH) at 2, 4, 6, 8, 10 and 12 month old were used to detect the mRNA expression in seven adipose tissues (great omental, small omental, tail, subcutaneous, mesenteric, perirenal and retroperitoneal fat) by real-time PCR. The results showed that PPARa and PPARy expressed in all tissues studied. Generally, the mRNA expressed lower in superficial fats than that in deep fat deposits. As the main effects, months of age and breed did not basically influence the mRNA expression of PPARa and PPARy. Though sex did not influence PPARa expression in both breeds, low expression of PPARy in females of GLT resulted in the significant sex effect and interaction between sex and breed. Based on the expression differences between tissues and ages, respectively, the expression of the two genes was of significant spatiality but not of temporality. The two genes had a synergistic effect on the metabolism of adipose tissues despite their opposite functions.关键词
绵羊/PPARα/PPARγ/脂肪组织/荧光定量PCRKey words
sheep/ PPARa/ PPARy-, adipose tissue/ real-time PCR分类
农业科技引用本文复制引用
林婄婄,刘文忠,高中元,袁亚男,武建亮,刘宝凤,周沙沙,乔利英,刘建华,梁琛..PPARα和PPARγ基因在不同脂尾型绵羊脂肪组织中的发育性表达研究[J].畜牧兽医学报,2012,43(9):1369-1376,8.基金项目
国家自然科学基金(30972084) （30972084）
山西省科技攻关项目(011029) （011029）
