畜牧兽医学报2012，Vol.43Issue(9)：1415-1421,7.
O型口蹄疫抗体金标检测试纸条判定标准的确立
Establishment of Judging Standard of Colloidal Gold-based Immunochromatographic Assay Strip for Detection Antibodes of Serotype O Foot-and-mouth Disease
摘要
Abstract
The objective of the study was to establish a judging standard of colloidal gold-based immunochromatographic assay strip (GICA) for detecting antibodies of serotype C) of foot-and-mouth disease (FMD) rapidly. The GICA of 3 batches were tested. There were a hundred and eighty FMD negative serum samples (containing pig, cattle and sheep), a hundred and ninety-seven FMD weak positive serum samples (containing pig, cattle), three hundred and seventeen FMD serotype O positive serum samples (containing pig, cattle and sheep), two hundred and twenty-three FMD serotype A and Asia 1 positive cattle sera, six hundred filed samples. To corn-pare three methods of GICA, liquid-phase competition ELISA (LB-ELISA) of FMDV serotype () and Indirect Hemagglutination Test (IHA) of FMDV serotype O for detecting antibodies of FMD serotype O, all the serum samples were detected, the judging standard was established. According to the color of test line and control line, compared with the titers of LB-ELISA, the colori-metric card was drawn. The judging standard of GICA was as follows: Using a 1 -.2 dilution of the sample, the result was negative, and the anti-FMDV serum was negative; Using a 1:8 dilution of the sample, the result was positive, which implied that the serum had serotype O FMDV positive antibodies. Compared with GICA and LB-ELISA, the colorimetric card was drawn which was helpful for reading results. The correlation of the GICA and LPBE was y = 1. 142a: + 0. 742 1; the correlation coefficient (r) was 0. 922 1; the correlation of the GICA and IHA was y = 1. 184 5x + 0. 862 3; the correlation coefficient (r) was 0. 903 3. These results indicated that the judging standard of GICA was prior to detecting antibodies of FMD serotype O; the colorimetric card was easy to read, which was just monitoring the anti-FMD serotype O antibodies of immunized animals fast and accurately. This judging standard could promote the qualitative analysis to semi-quantitative analysis, and it had provided that it was more suitable for using in laboratory and the field.关键词
O型口蹄疫抗体金标检测试纸条/O型口蹄疫液相阻断ELISA/O型口蹄疫正向间接血凝/判定标准/比色卡Key words
colloidal gold-based immunochromatographic assay strip of FMDV serotype O/ liquid-phase competition ELISA of FMDV serotype O/ indirect hemagglutination test of FMDV serotype O/ judging standard/ colorimetric card分类
农业科技引用本文复制引用
智晓莹,任维维,祁光宇,吕建亮,梁仲,蒋韬..O型口蹄疫抗体金标检测试纸条判定标准的确立[J].畜牧兽医学报,2012,43(9):1415-1421,7.基金项目
甘肃省科技厅资助 （）
甘肃科技重大技术专项(1002NKDA037) （1002NKDA037）
