畜牧兽医学报2012，Vol.43Issue(9)：1449-1454,6.
表达鸡传染性支气管炎病毒S1蛋白重组腺病毒的构建及免疫原性的初步分析
Construction and Immunogenicity Detection of Recombinant Adenovirus Expressing S1 Gene of Infections Bronchitis Virus
宋菲菲 1康震 2齐艳君 3袁颖烁 3张守峰 1张乐萃 2扈荣良2
作者信息
- 1. 青岛农业大学动物科技学院,青岛266109
- 2. 军事医学科学院军事兽医研究所,长春130122
- 3. 寿光六和肉鸡养殖有限公司,潍坊262700
- 折叠
摘要
Abstract
To construct human adenovirus type 5 vectored recombinant virus expressing S1 glyco-protein gene of avian infectious bronchitis virus (IBV), S1 gene of IBV QS10 strain obtained through RT-PCR was inserted into shuttle plasmid of the adenoviral expression system, forming pacAd5 CMV-S1, as well as the backbone plasmid, which was linearized with Pac I and trans-fected into 293AD cells. Detection with PCR revealed that the recombinant virus was successfully packaged with a titer up to 107TCID50 o ml-1. Furthermore, The results of Western blotting and indirect immunofluorescence assay (IFA) indicated that the SI gene was significantly expressed. Sera antibodies against IBV of the SPF chicken immunized with the recombinant virus were detected by enzyme-linked immunosorbent assay (ELISA). The recombinant adenovirus expressing SI gene of IBV was successfully constructed with potent capacity to induce the specific antibodies in chickens and had the good immunogenicity, which laid the foundation for further development of recombinant avian infectious bronchitis vaccine.关键词
鸡传染性支气管炎病毒/S1蛋白/人5型腺病毒载体/免疫原性Key words
infectious bronchitis virus/ SI protein/ human adenovirus type 5/ immunogenicity分类
农业科技引用本文复制引用
宋菲菲,康震,齐艳君,袁颖烁,张守峰,张乐萃,扈荣良..表达鸡传染性支气管炎病毒S1蛋白重组腺病毒的构建及免疫原性的初步分析[J].畜牧兽医学报,2012,43(9):1449-1454,6.
