畜牧兽医学报2012，Vol.43Issue(9)：1463-1470,8.
共轭亚油酸对堆型艾美耳球虫感染鸡十二指肠黏膜免疫和脂质过氧化状态的影响
Effects of Dietary Conjugated Linoleic Acid on the Duodenumal Mucosal Immunity Response and Lipid Peroxidation of Broiler Chicks Infected with E.aceruvlina
摘要
Abstract
The present experiment was conducted to investigate effects of conjugated linoleic acid (CLA) on growth performance, oocyst shedding, duodenumal mucosal immune response and lip-id peroxidation of broiler chicks infected with E. aceruvlina. After 21 days of dietary supplementation with either basal diets or 1. 5% CLA diets, half of replicates in each treatment were inoculated with 10 000 E. aceruvlina sporulated oocysts. Compared with basal diets, 1. 5% CLA diets ameliorated growth inhibition (P＜0. 01＝ , and decreased oocyst shedding between 6-9 d post-inoculation resulted from E. aceruvlina infection (P＜0. 01＝. Whether infection or not, duodenumal intraepithelial lymphocyte (IEL) CD8+T lymphocyte subpopulations were significantly higher in chicks fed CLA diet than the controls (P＜0. 05＝, but interferon γ (IFNγ) gene expression was not (P＞0. 05). Dietary CLA notably suppressed the increase of MDA in duodenumal mucosa (F＜0. 05＝, and inhibited the decrease of glutathione (GSH) concentration (P＜0. 01＝ resulted from E. aceruvlina infection, but didn; t influence the activity of total superoxide dismutase (TSOD) and catalase (CAT)(P＞0. 05). Dietary CLA decreased oocyst shedding and ameliorated growth ihibition of broiler chicks resulted from E. aceruvlina infection at least in part through increasing duodenumal IEL CD8+ T lymphocyte subpopulations and inhibiting the decrease of duo-denumal mucosal GSH in broiler chicks.关键词
共轭亚油酸/堆型艾美耳球虫/肉仔鸡/肠道黏膜免疫/氧化应激Key words
CLA/ Eimeria aceruvlina/ broiler chicks/ intestinal mucosal immune/ oxidative stress分类
农业科技引用本文复制引用
刘永祥,杨建平,姜东风,刘艳丽,徐秋良..共轭亚油酸对堆型艾美耳球虫感染鸡十二指肠黏膜免疫和脂质过氧化状态的影响[J].畜牧兽医学报,2012,43(9):1463-1470,8.基金项目
河南省科技厅重点科技公关资助项目(082102110123) （082102110123）
郑州牧专博士基金项目(2007DSR003) （2007DSR003）
