畜牧兽医学报2012，Vol.43Issue(9)：1471-1478,8.
中国主要疯草内生真菌分离鉴定及其抑菌活性研究
Study on Isolation, Identification and the Antibacterial Activity of Endophytic Fungi from the Major Locoweed in China
摘要
Abstract
The objective of this study was to ascertain the species, distribution and genetic development of the endophytic fungus from the major locoweed in the west meadow of China and detect the antibacterial activity of their secondary metabolites. The study collected Oxytropis flabra , Oxytropis Ochrocephala Bunge , Oxytropis Kansuensis , Oxytropis Deflexa , Oxytropis Coer-ulea and Astragalus variabilis samples to isolate and purify the endophytic fungi in the different tissues, then used the morphology techniques and ITS sequence analysis to identify their species, and built the phylogenetic tree by Neighbor-Joining method. Staphylococcus aureus , Bacillus subtilis , Escherichia coli and Salmonella were selected as indicators to do the antibacterial tests of the fungi secondary metabolites by paper disc diffusion method. 265 fungal colonies were isolated from the collected locoweed, which were combined to 38 kinds of different endophytic fungus. The distribution of the endophytic fungus in the tissues of locoweed was different; Fusarium sp.Alternaria sp. and Paecilomyces sp. were the dominant strains, which occupied 16. 98%, 11. 33% and 9. 81% of the total isolated colonies respectively. Phylogenetic analysis showed that 38 kinds of endophytic fungus could be divided into two populations based on the evolutionary kinship. The endophyte species and the distance of genetic relationship belonged to locoweed species, tissues, ecological environment and other factors. The antibacterial tests results showed that the secondary metabolites of 29 strains at least (76. 32% of the total strains isolated) presented the antibacterial activity against one of the tested bacteria, of which the secondary metabolites of two strains were found to have stronger antibacterial activity against the four tested bacteria. There are various species of endophytic fungus in the major locoweed in the west meadow of China, the endophytes in different ecological environment have a certain kinship but also with some differences. Diverse antibacterial natural products are existed in the secondary metabolites of endophytic fungus from locoweed, which could be a new resource to screen active substance with antibacterial activity.关键词
疯草/内生真菌/分离/鉴定/抑菌活性Key words
locoweed/ endophytic fungi/ isolation/ identification/ antibacterial activity分类
农业科技引用本文复制引用
陈基萍,赵宝玉,路浩,丁来弟,张广芳,李小蒙,高源,周启武,杨晓雯..中国主要疯草内生真菌分离鉴定及其抑菌活性研究[J].畜牧兽医学报,2012,43(9):1471-1478,8.基金项目
国家自然科学基金项目(31072175 （）
31201958) （）
国家"十二五"公益性行业(农业)科研专项(201203062) （农业）
教育部高校博士点基金新教师项目(20100204120018) （20100204120018）
中央高校基本科研业务费(QN2011108) （QN2011108）
