畜牧兽医学报2012，Vol.43Issue(9)：1491-1498,8.
成年版纳微型猪近交系克隆猪的制备
Production of Cloning Adult Banna Miniature Inbred Pig
摘要
Abstract
Fibroblast cells were isolated from adult Banna Miniature Inbred pig with cold trypsin digestion method and subcultured. Through study of cell biology characteristics, effects of fibroblast cells as the donor cells on somatic cell nuclear transfer was investigated. The results showed that isolated fibroblast cells were morphologically consistent with typical fibroblast, cell viability was 97. 2% before freezing and 92. 6% after thawing (P＞0. 05). The cell growth curve was 'S' shape and population doubling time was approximately 36 h. The karyotype was normal after 14 passages of subculture in vitro. When isolated fibroblast cells were used as donor cells for somatic cell nuclear transfer, the cleavage rate, the blastocyst rate and cell number of blastocyst were 61. 9%, 13. 0% and 37, respectively. One of three surrogate pigs after somatic cell nuclear transfer with fibroblast cells as donor cells was pregnant and gave birth to one clone pig. In brief, fibroblast cell line could be established from adult Banna Miniature Inbred pig. The isolated fibro- blast cells could be used as donor cells for somatic cell nuclear transfer and gave birth to the clone pig of Banna Miniature Inbred pig.关键词
版纳微型猪近交系/成年猪成纤维细胞/细胞培养/生物学特征/体细胞核移植Key words
Banna Miniature Inbred pig/fibroblast/cell culture/biological characteristics/ somatic cell nuclear transfer分类
农业科技引用本文复制引用
叶雷,信吉阁,李思,魏太云,李小兵,张崇义,汪霞,曾养志,李红,魏红江,许成盛,卿玉波,潘伟荣,李鸿辉,成文敏,富国文..成年版纳微型猪近交系克隆猪的制备[J].畜牧兽医学报,2012,43(9):1491-1498,8.基金项目
国家自然科学基金项目(31060308) （31060308）
973计划项目(2011CB944200) （2011CB944200）
