畜牧兽医学报2012，Vol.43Issue(10)：1531-1538,8.
鸡前脂肪细胞中A-FABP基因表达的变化对PPARγ、perilipin和E-FABP表达的影响
The Effect of A-FABP Gene Overexpression or Knockdown on the Expression of PPARγ, perilipin and E-FABP in Chicken Adipocytes
摘要
Abstract
This study was designed to investigate the role of chicken A-FABP gene in lipid metab-olism of adipocytes and the regulation relationship between AFABP gene and the genes related to lipid metabolism. The shRNA interference vector with A-FABP gene and eukaryotic expres-sion vector were used. Chicken adipocytes with short hairpin RNA-mediated knockdown and A-FABP cDNA overexpression were used. The genes expression level in chicken adipocytes were detected at 24, 36, 48, 60 and 72 h after transfection with the interference and overexpression plasmids of AFABP. The results showed that the expression levels of PPARy and perilipin genes were downregulated at 24 and 36 h and upregulated at 48 h in the A-FABP interference group. The expression levels of E-FABP genes was downregulated at 48 and 72 h in the A-FABP interference group. The expression levels of PPARy and perilipin genes were upregulated at 24 and 36 h and downregulated at 48 h in the A-FABP overexpression group. The expression levels of E-FABP genes was downregulated at all detected time pionts in the A-FABP overexpression group. These results indicated that A-FABP might affect expression of PPARy, perilipin and E-FABP in chicken adipocytes.关键词
A-FABP基因/PPARγ基因/perilipin基因/E-FABP基因/鸡/脂肪细胞Key words
A-FABP gene/ PPARy gene/ perilipin gene/ E-FABP gene/ chicken/ adipocytes分类
农业科技引用本文复制引用
张庆秋,石慧,王宇祥,李辉,王启贵..鸡前脂肪细胞中A-FABP基因表达的变化对PPARγ、perilipin和E-FABP表达的影响[J].畜牧兽医学报,2012,43(10):1531-1538,8.基金项目
国家自然科学基金项目(30771542 （）
30972087) （）
现代农业产业技术体系建设项目(CARS-42)黑龙江省高等学校科技创新团队建设项目(2010td02) （CARS-42）
