畜牧兽医学报2012，Vol.43Issue(10)：1539-1546,8.
绒山羊骨骼肌miRNAs及其靶基因表达谱分析
The Analysis of miRNAs and Their Target Expression Profiles in Cashmere Goat Skeletal Muscle
摘要
Abstract
Seven muscle and adipose related miRNAs, including miR-1, miR-133, miR-24, miR-122, miR-103, miR-143 and Iet7 were selected to analyze their expression and target expression profiles. Muscles RNA from back and hind leg muscle of 4 cashmere goats were extracted, after reverse-transcription, SYBR TaqMan PCR was used to detect gene expression, and miRNA tar-get expression profiles were got by PAGE electrophoresis and MiRNA Targets Figure Print (MTFP) technique. The result showed that the expression of miRNA were as follows: miR-1 > miR-133>miR-24>let7> miR-143 > miR-103 >miR-122, the expression patterns of miR-1 and miR-133 were similar,and the expression levels of all miRNAs in adult goat were higher than that in lamb; moreover, the difference of miRNAs expression levels were also affected by gender, and they had relationship with number and expression levels of target genes. 10 target genes in 5 miRNAs were sequenced and Blast annotation, which coded proteins related to signal transduc-tion, cell cycle, and so on. The expression analysis of 7 miRNAs and their target genes showed differences and similarities between muscle development related miRNAs and adipose develop-ment related miRNAs in cashmere goat skeletal muscle, which were favorable to the research for meat quality related genes in cashmere goat.关键词
miRNA/靶基因/表达/绒山羊/骨骼肌Key words
miRNA/ target gene/ expression/ cashmere goat/ skeletal muscle分类
农业科技引用本文复制引用
韩志玲,赵德超,付绍印,吕晓曼,高爱琴,张文广,李金泉..绒山羊骨骼肌miRNAs及其靶基因表达谱分析[J].畜牧兽医学报,2012,43(10):1539-1546,8.基金项目
国家科技支撑项目(2011BAD28B05) （2011BAD28B05）
国家自然科学基金(30960246) （30960246）
现代农业产业技术体系建设专项资金(CARS-40-05) （CARS-40-05）
公益性行业(农业)科研项目(201003019) （农业）
