畜牧兽医学报2012，Vol.43Issue(10)：1639-1644,6.
猪链球菌-副猪嗜血杆菌二联亚单位疫苗在小鼠的免疫效力分析
Analysis of Immune Effect on Streptococcus suis-Haemophilus parasuis Bivalent Subunit Vaccine in Mice
摘要
Abstract
This study aims to develop a new kind of bivalent genetic engineering vaccine used to control Streptococcus suis (SS) disease and Haemophilus parasuis (HPS) disease, and to evaluate the vaccine's immune effect. Four proteins, Streptococcus suis serotype 2 proteins SLY, HP0197, and Haemophilus parasuis outer membrane proteins D15 and HPS06257 are selected, recombinant expressed, purified and added adjuvant of certain proportion to be made with different components of subunit vaccines. In the present study, the Streptococcus suis serotype 2-Hae-mophilus parasuis (SS2-HPS) bivalent subunit vaccine and their single vaccines could elicit a significant humoral antibody response and confer significant protection against challenge with lethal dose of SS2 or HPS in mice, respectively. In addition, the hyperimmune sera against these vaccines could efficiently kill the bacteria in the opsonized phagocytosis test and confer significant protection against SS2 infection in the experiment of passive immunization. The study shows this SS-HPS bivalent subunit vaccine has good immune protection in mice.关键词
猪链球菌2型/副猪嗜血杆菌/二联亚单位疫苗/调理吞噬试验/免疫保护Key words
Streptococcus suis serotype 2/ Haemophilus parasuis / bivalent subunit vaccine/ opsonized phagocytosis test/ immunoprotection分类
农业科技引用本文复制引用
赵建平,魏燕鸣,宋岱松,张强,朱剑,周明光,李冉,康超,金梅林..猪链球菌-副猪嗜血杆菌二联亚单位疫苗在小鼠的免疫效力分析[J].畜牧兽医学报,2012,43(10):1639-1644,6.基金项目
国家863计划(2011AA10A210) （2011AA10A210）
国家973计划(2012CB518805) （2012CB518805）
