畜牧兽医学报2012，Vol.43Issue(10)：1645-1650,6.
大熊猫等8种野生哺乳动物蛔虫的线粒体COXⅠ和COXⅡ基因的亲缘关系分析
Genetic Relationship of Ascarid Nematodes from Giant Panda and Seven Other Species of Captive Wild Mammals Based on COX Ⅰ and COX Ⅱ Genes
摘要
Abstract
The aim of this study was to investigate the taxonomic relationships of ascarid nema-todes from Ailuropoda melanoleuca , Ailurus fulgens , Ursus maritimus , Ursus arctos pruino-sus , Ursus thibetanus mupinensis , Ursus arctos lasiotus , Pan troglodytes and Hylobates hoolock. The mitochondrial cytochrome coxidase subunits Ⅰ (COX Ⅰ ) and Ⅱ (COXⅡ ) were amplified by PCR and sequenced directly. Then, sequence analyses was carried out based on COX Ⅰ and COX Ⅱ sequences from ascarid nematodes acquired in our study and 12 homologous sequences from GenBank. The lengths of COX I and COX H in ascarid nematodes from the eight species of cap-tive animals were all 393 bp and 582 bp, respectively. The COX I and COX II of ascarid nema-todes from giant panda shared 94. 8%-95. 0% and 94. 9%-95. 5% sequence identity with these from A. fulgens, U. maritimus, U. arctos pruinosus , U. thibetanus mupinensis and U. arctos lasiotus, respectively. Additionally, the COX Ⅰ and COXⅡ genes of ascarid nematodes from P. troglodytes shared 99. 8% and 99. 5% identity with the nematodes from H. hooloc, respectively. Phylogentic analysis showed that ascarid nematodes from A. melanoleuca, A. fulgens and four Ursids species belonged to the genus Baylisascaris, while roundworms from H. hoolock and P. troglodytes clustered under the genus Ascaris. These results showed that a co-evolutionary rela-tionship may link the ascarid nematodes and their corresponding hosts together.关键词
大熊猫/野生哺乳动物/蛔虫/线粒体/COXⅠ/COXⅡ/亲缘关系Key words
giant panda/ wild mammals/ ascarid nematodes/ mitochondria/ cytochrome coxidase subunit Ⅰ (COX Ⅰ ) / cytochrome coxidase subunit Ⅱ (COX Ⅱ ) / genetic relationship分类
农业科技引用本文复制引用
牛李丽,陈维刚,王淑贤,杨光友,陈世界,汪涛,古小彬,严玉宝,余华,邓家波,严慧娟,余星明..大熊猫等8种野生哺乳动物蛔虫的线粒体COXⅠ和COXⅡ基因的亲缘关系分析[J].畜牧兽医学报,2012,43(10):1645-1650,6.基金项目
成都大熊猫繁育研究基金项目(CPF-08005) （CPF-08005）
科技部公益项目(200910188) （200910188）
国家质检总局项目(2010IK004) （2010IK004）
