畜牧兽医学报2012，Vol.43Issue(10)：1651-1656,6.
负载重组口蹄疫病毒VP4蛋白的树突状细胞启动的T细胞应答
T Cell-mediated Responses Initiated by Dendritic Cells Pulsed with Recombinant VP4 Protein of Foot-and-mouth Disease Virus
摘要
Abstract
This study was designed to seek the activation effect on T lymphocytes by dendritic cells pulsed with recombinant VP4 protein of foot-and-mouth disease virus (FMDV). To this end, we have successfully constructed the prokaryotic expression vector of pET32a-VP4. The recombinant VP4 protein was expressed with induction of IPTG. VP4 protein was purified by SDS-PAGE and electro-elution approaches and identified with Western blot. Bone morrow-derived dendritic cells (BMDCs) were generated in vitro and pulsed with purified VP4 protein. The VP4-loaded BMDCs were co-cultured with lymph node T cells. Culture supernatants were harvested at indicated time points and the levels of IFN-y were assayed with ELISA. The results showed that the IFN-y levels of test groups were higher than that of control groups significantly at 3, 9, and 48 hours after co-culture. These data indicate that BMDCs pulsed with FMDV VP4 protein are a-ble to efficiently stimulate T lymphocyte activation and consequently initiate Thl-like response with enhanced secretion of IFN-?.关键词
口蹄疫病毒/VP4蛋白/原核表达/树突状细胞/T细胞Key words
foot-and-mouth disease virus/ VP4 protein/ prokaryotic expression/ dendritic cell/ T cell分类
农业科技引用本文复制引用
安鹏丽,李娜,李丽敏,张丽,张雷,高云欢,王家鑫..负载重组口蹄疫病毒VP4蛋白的树突状细胞启动的T细胞应答[J].畜牧兽医学报,2012,43(10):1651-1656,6.基金项目
国家自然科学基金资助项目(30972168) （30972168）
