畜牧兽医学报2012，Vol.43Issue(11)：1767-1772,6.
广东猪输血传播病毒流行病学调查及其主要抗原编码区进化分析
Epidemiological Investigation and Antigen Phylogeny Analyses in Guangdong for Porcine Transfusion Transmitted Virus
摘要
Abstract
The objective of this study was to investigate the prevalence of porcine transfusion transmitted virus (TTV) species (TTSuVl and TTSuV2). The study was conducted in 7 different intensive pig farms of Guangdong province in China. A total of 159 swine sera were collected, and then the virus were tested using Nest-PCR. The results demonstrated that the TTSuVl and TTSuV2 infection rates were 54. 72% and 34. 22% respectively, and the co-infection rate was 29. 56%. We designed PCR primers based upon the conserved region of the two viruses for amplifying the two virogenes. By blast analysis with our cloned TTsuVs sequences in NCBI database, we found that our isolated TTsuVl ORFland TTsuV2 ORF1, have high homologousto to those known TTSuVs sequences, 72%-75% and 88%-97% respectively; while have big gaps with the TTVs which infecting other species like feils, canis, tupaia, tamarin, douroucouli and homo in the nucleotide sequence. To sum up, TTSuVs had been widely distributed in Guangdong's pig populations. This study laid the foundation for further research of the prevalence situation, serologic diagnostic methods and to prepare for safe and valid vaccine.关键词
TTSuV1/TTSuV2/流行病学/抗原进化分析Key words
Torque teno sus virus 1/ Torque teno sus virus2/ epidemiology/ antigen evolutionary analysis分类
农业科技引用本文复制引用
陈轶雯,李中圣,白挨泉,陈善真,李其昌,姚火春,王贵平..广东猪输血传播病毒流行病学调查及其主要抗原编码区进化分析[J].畜牧兽医学报,2012,43(11):1767-1772,6.基金项目
广东省科技厅项目(2011A090400011) （2011A090400011）
