畜牧兽医学报2012，Vol.43Issue(11)：1773-1779,7.
1株猪繁殖与呼吸综合征病毒ORF5基因缺失毒株的基因组特征与演化分析
Genomic Characterization and Evolvement Analysis of a Porcine Reproductive and Respiratory Syndrome Virus Isolate with Deletion in GP5
摘要
Abstract
In order to understand the variation of porcine reproductive and respiratory syndrome virus (PRRSV) in Shaanxi province, the virus was detected from swine clinical samples, NSP2 and ORF5 genes were amplified, cloned and sequenced for analyzing the genetic variation of PRRSV. The result showed that the strain belonged to the North American genotype, named HZ1007. It had discontinuous 30 amino acids deletion in the aa position of 481 and 533-561 of NSP2, which was the same deletions with the highly pathogenic PRRSV. Moreover, the ORF5 gene of HZ1007 strain contained a continuous 11 amino acids deletion in the aa positon of 85-95. PRRSV HZ1007 was highly homologous to HUN4 and SY0608 strains. Phylogenetic tree analysis revealed that HZ1007 had closer relationship with the highly pathogenic PRRSV than classical strains, it may be a new variant of the highly pathogenic strain. The athogenic force and biological characteristics needed further research and analysis关键词
猪繁殖与呼吸综合征病毒/NSP2基因/GP5基因缺失/序列分析Key words
porcine reproductive and respiratory syndrome virus/ NSP2 gene/deletion in GF5 gene/ sequence analysis分类
农业科技引用本文复制引用
付向晶,贺生芳,杜恩岐,杨增岐,王丹阳,王兴龙,张渭东,宋祥军,刘阳坤,唐文雅,慕国辉,刘海金..1株猪繁殖与呼吸综合征病毒ORF5基因缺失毒株的基因组特征与演化分析[J].畜牧兽医学报,2012,43(11):1773-1779,7.基金项目
"十一五"国家科技支撑计划项目(2006BAD06A08) （2006BAD06A08）
