鸡传染性支气管炎病毒S1蛋白抗原表位的串联表达及间接ELISA方法的建立OA北大核心CSCDCSTPCD
Development of an Indirect ELISA of Infectious Bronchitis Virus by Using Tandem Epitopes of S1
本研究旨在建立一种快速、简便的鸡传染性支气管炎抗体的检测方法.通过生物信息学软件对传染性支气管炎病毒(IBV) ZY3株的S1蛋白进行分析后,筛选出4段S1蛋白抗原表位优势区域(FI～F4),将4段表位串联成1条新基因F,对F基因编码的蛋白质进行二级结构预测·结果表明该蛋白抗原指数性高且具有良好的柔韧性.构建重组表达载体pET-32a(+)-F,并在原核表达系统中表达,获得大小为42 ku的融合蛋白,经Western blot 分析表明表达的串联…查看全部>>
The aim of this study was to establish an indirect ELISA for detection of antibodies against avian infectious bronchitis (IB). The published amino acid sequences of SI gene of avian Infectious bronchitis virus (IBV) strain ZY3 were analyzed by bioinformatics software, and four dominant epitopes named as Fl, F2, F3 and F4 were selected and ligated together as a chimeric gene F. Bioinformatic analysis showed that this protein if highly antigenic and flexible. …查看全部>>
孙罗美;易林;邹年莉;柳萍;黄勇
四川农业大学动物医学院,雅安625014四川农业大学动物医学院,雅安625014四川农业大学动物医学院,雅安625014四川农业大学动物医学院,雅安625014四川农业大学动物疫病与人类健康四川省重点实验室,雅安625014
农业科技
传染性支气管炎病毒S1蛋白表位串联间接ELISA建立
Infectious bronchitis virus (IBV) SI protein tandem epitopes indirect ELISA deveiope
《畜牧兽医学报》 2012 (11)
1780-1787,8
教育部《长江学者和创新团队发展计划》创新团队项目(IRTO848)
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