畜牧兽医学报2012，Vol.43Issue(11)：1780-1787,8.
鸡传染性支气管炎病毒S1蛋白抗原表位的串联表达及间接ELISA方法的建立
Development of an Indirect ELISA of Infectious Bronchitis Virus by Using Tandem Epitopes of S1
摘要
Abstract
The aim of this study was to establish an indirect ELISA for detection of antibodies against avian infectious bronchitis (IB). The published amino acid sequences of SI gene of avian Infectious bronchitis virus (IBV) strain ZY3 were analyzed by bioinformatics software, and four dominant epitopes named as Fl, F2, F3 and F4 were selected and ligated together as a chimeric gene F. Bioinformatic analysis showed that this protein if highly antigenic and flexible. Then the chimeric gene was then inserted into expression vector pET-32a( + )for the expression of target gene and a 42 kD recombinant protein was obtained. The result of western-blot showed that the chimeric protein could react specifically with anti-IBV positive serum. An indirect ELISA was then developed using purified protein as coating antigen. 175 sera samples were examined by this ELISA and commercial kit, the results showed that the positive coincidence rate could reached 90.2%, negative coincidence rate could reached 85. 7%, and the total coincidence rate reached 89. 7%. The results indicated that the indirect ELISA was sensitive and specific, and no cross-reaction with positive sera of other chicken diseases. The indirect ELISA for detection of chickenantibodies against Infectious bronchitis were successfully developed关键词
传染性支气管炎病毒/S1蛋白/表位串联/间接ELISA/建立Key words
Infectious bronchitis virus (IBV) / SI protein/ tandem epitopes/ indirect ELISA/ deveiope分类
农业科技引用本文复制引用
孙罗美,易林,邹年莉,柳萍,黄勇..鸡传染性支气管炎病毒S1蛋白抗原表位的串联表达及间接ELISA方法的建立[J].畜牧兽医学报,2012,43(11):1780-1787,8.基金项目
教育部《长江学者和创新团队发展计划》创新团队项目(IRTO848) （IRTO848）
