畜牧与兽医2012，Vol.44Issue(11)：1-4,4.
牛副流感病毒3型核衣壳蛋白基因的真核表达
Eukaryotic expression of nucleocapsid protein gene from bovine parainfluenza virus type 3
摘要
Abstract
The aim of this study is to construct an eukaryotic expression plasmid for nucleocapsid protein ( N) gene of bovine parainfluenza virus type 3 ( BPIV-3 ). According to the complete sequence of BPIV-3 NM09 strain ( GenBank accession: JQ063064 ) , a pair of primers was designed and synthesized. The N gene of BPIV3 was amplified by reverse transcription polymerase chain reaction (RT-PCR). The N gene was cloned into pcDNA3. 1 (+) vector using Nhel and Notl, and thus the expression plasmid pcDNA3. 1-N was successfully constructed. Then pcDNA3. 1-N was transfected into BSR cell using lipoplast mediate method and positive results were demonstrated by indirect im-munofluorescence assay (IFA) and RT-PCR. The results showed N gene of BPIV-3 was successfully expressed in BSR cell. The study provided fundamental information for the further study on the prevention of BPIV3 infection and new vaccine development关键词
牛副流感病毒3型/真核表达/N基因Key words
bovine parainfluenza virus type 3/ eukaryotic expression/ N gene分类
农业科技引用本文复制引用
师新川,温永俊,王凤雪,王炜,王建科,程世鹏,武华..牛副流感病毒3型核衣壳蛋白基因的真核表达[J].畜牧与兽医,2012,44(11):1-4,4.基金项目
国家"863"项目(2011AA10A213). （2011AA10A213）
