西南国防医药2012，Vol.22Issue(10)：1045-1047,3.DOI:10.3969/j.issn.1004-0188.2012.10.001
PD98059 抑制快速起搏诱导心房肌细胞离子通道表达变化的研究
Effects of inhibition of PD98059 on expression changes of rapid pacing induced atrial muscle cell ion channel
摘要
Abstract
Objective To establish the rapid pacing model with the primarily cultured atrial muscle cell, and to study the effects of PD98059 (inhibitor of ERK1/2) on the expression of L - type calcium channel and potassium channel Kv4?3 in the early stage of rapid pacing. Methods Primarily cultured rats atrial muscle cells were used to establish rapid pacemaker cell model. These cells were randomly divided into three groups, i. e. control group, rapid pacing group, and PD98059 plus rapid pacing group. RT -polymerase chain reaction ( RT - PCR) and Western blot method were used to detect the changes of the expression of mRNA and protein in L - type calcium channel ale and potassium channel Kv4. 3 under different circumstances. Results After the rapid pacing for 24 h, compared with the expression of mRNA and protein in the control group, these in L - type calcium channel ale significantly decreased. Pretreatment with PD98059 before the pacing could significantly inhibit the decrease of the mRNA and protein expression in the rapid pacing - induced L - type calcium channel ale and potassium channel Kv4.3(P < 0. 01). But the expression levels were still lower than these of the control group (P <0. 05). Conclusion In the early stage of rapid pacing, the expression of mRNA and protein in L - type calcium channel otic and potassium channel Kv4. 3 decreases in different degrees, which indicates that the reconstruction of ion channel occurs. And the inhibitor PD98059 of ERK1/2 can significantly inhibit the decrease of the expression, which indicates that ERK1/2 takes part in the reconstruction of atrial muscle cell ion channel in the early stage of rapid pacing.关键词
心房肌细胞/快速起搏/离子通道/PD98059/重构Key words
atrial muscle cell/ rapid pacing/ ion channel/ PD98059/ reconstruction分类
医药卫生引用本文复制引用
程伟,朱昀,肖颖彬..PD98059 抑制快速起搏诱导心房肌细胞离子通道表达变化的研究[J].西南国防医药,2012,22(10):1045-1047,3.基金项目
国家自然科学基金资助(30600252) （30600252）
