西南国防医药2012，Vol.22Issue(11)：1168-1170,3.DOI:10.3969/j.issn.1004-0188.2012.11.003
HPLC法测定紫果西番莲叶中异牡荆素的含量
Detection of isovitexin content in leaves of passiflora edulis by high performance liquid chromatography
摘要
Abstract
Objective To detect the content of isovitexin in the leaves of Passiflora edulis by high performance liquid chromatography ( HPLC ). Methods Firstly, the extraction and purification were carried out by silica gel column chromatography. HPLC separation was carried out in Hypersil ODS C_18 column ( 4. 6 × 250 mm, 5μm ) with the mobile phase of acetonitrile - 0. 1% H_3PO_4 solution (18: 82 ), and the flow rate was 1. 0 ml/min. The sample size was 10 μl, and the detection wavelength was 350 nm. Results Within the range of 0.012 - 0. 120 mg/ml, isovitexin had a good linear relationship ( R =0. 997 ). The average recovery was 98. 26% , and RSD was 1. 4% . The average content of isovitexin was 0. 0873% . Conclusion The method is accurate, reliable, and repeatable, and can be used to detect the content of isovitexin in the leaves of passiflora edulis.关键词
紫果西番莲叶/异牡荆素/HPLCKey words
leave of passiflora edulis/ isovitexin/ high performance liquid chromatography分类
医药卫生引用本文复制引用
孔秋玲,赵瑞瑞,邹江冰,蒋琳兰..HPLC法测定紫果西番莲叶中异牡荆素的含量[J].西南国防医药,2012,22(11):1168-1170,3.基金项目
广东省医院药学研究基金(奥赛康基金)(2011A23) （奥赛康基金）
广州市委基础研究计划项目(2005J1-C0352) （2005J1-C0352）
