烟草科技Issue(10)：77-80,4.
烟草普通花叶病毒抗血清的吸附纯化及应用
Adsorption Purification of TMV Antiserum and Detection Application
摘要
Abstract
For the detection of TMV in tobacco, antiserum was prepared by injecting purified TMV virion into mouse and the titer of antiserum was determined after adsorption purification, an ELISA analysis system was established basing on the in-house TMV antiserum. The results of PAGE indicated the extracted TMV virion was of high purity and concentration; non-specific antibody could be effectively diminished by adsorption with tobacco total protein; the titer of antiserum was as high as 1:40960; the concentrations of reagents in ELISA detection optimized by orthogonal continuous dilution method were 1:20000 in dilution for secondary antibody, 1:2000 for TMV antiserum and 1:20 for sample supernatant.关键词
烟草普通花叶病毒(TMV)/抗血清/吸附纯化/ELISAKey words
TMV/Antiserum/Adsorption purification/ELISA分类
农业科技引用本文复制引用
罗朝鹏,魏春阳,李乃会,杨军,金大伟,金立锋,李峰,林福呈..烟草普通花叶病毒抗血清的吸附纯化及应用[J].烟草科技,2012,(10):77-80,4.基金项目
郑州烟草研究院科技项目"烟草主要病毒病分子变异监控及追踪技术研究"(122009CZ0410) （122009CZ0410）
郑州烟草研究院院长科技发展基金项目"烟草香气前体物代谢途径关键酶基因DXS克隆及表达特征研究"(902011CA0330). （902011CA0330）
