烟草科技Issue(11)：45-50,6.
LC-MS/MS同时检测烟草中的6种杀菌剂
Simultaneous Determination of 6 Antiseptics in Tobacco with LC-MS/MS Method
摘要
Abstract
A method basing on high performance liquid chromatography-tandem mass spectrometry (LC-MS/ MS) was developed for determining the antiseptic residues in tobacco. The analytes extracted from tobacco samples were separated on C18 column (150 mm×2.1 mm×3 μm) using acetonitrile-water (containing 0.1% formic acid) as mobile phase. The antiseptics were determined by a tandem mass spectrometer with internal standard under multiple reaction monitoring (MRM)mode. The 145 domestic and imported tobacco samples were determined with this method. The results showed that: 1)The linear range was 5-200 ng/mL (/R2>0.999). The average recoveries ranged from 85.3% to 118.8% with the relative standard deviations (RSD) less than 7.7%. 2)No antiseptic residue was detected in 52 tobacco samples and 30 cigarette samples, which accounted for about 52% of all leave samples and 66.7% of all cigarette samples; all the mentioned antiseptics, except benomyl, were detectable in samples. This method was suitable for the fast determination of the residues of the 6 antiseptics in tobacco and tobacco products.关键词
杀菌剂/液相色谱-串联质谱法(LC-MS/MS)/烟草Key words
Antiseptic/LC-MS/MS/Tobacco分类
轻工纺织引用本文复制引用
杨飞,边照阳,唐纲岭,胡清源..LC-MS/MS同时检测烟草中的6种杀菌剂[J].烟草科技,2012,(11):45-50,6.基金项目
国家烟草专卖局标准项目"烟草及烟草制品 农残限量要求"(项目编号 502011AB0450)和中国烟草总公司郑州烟草研究院院长基金项目(512012CA0080). （项目编号 502011AB0450）
