印染助剂2012，Vol.29Issue(12)：11-14,4.
酸的种类、pH和温度对酸性纤维素酶活力的影响
Effects of acids, pH and temperature on acid cellulase activity
摘要
Abstract
Eight kinds of acids (formic acid, acetic acid, glycoiic acid, lactic acid, citric acid, tartaric acid; glycine and aminosulphonic acid) were used as buffer solutions with Ph=5.0. The acid cellulase activities were measured individually in these buffer solutions. Meanwhile using acetic acid as Ph regulator, the effects of Ph and temperature on cellulose activity were analyzed and optimized by central composite design to obtain linear regression equatian and optimal factors. The results showed that tartaric acid and lactic acid exhibited higher activity than acetic acid at Ph 5.0, glycoiic acid, formic acid and citric acid gave little lower activity compared with acetic acid, while the glycine, aminosulphonic acid gave much lower cellulase activity than acetic acid. Applying acetic acid to adjust Ph, cellulase activity increased with the rising of temperature and the decline of Ph. Ph showed more significance than temperature, the optimum performance of activity was at 49.8 ℃,Ph= 4.8.关键词
酸性缓冲液/纤维素酶活力/线性回归/中心合成设计法Key words
acid buffer solution/ cellulase activity/ linear regression/ central composite design分类
化学化工引用本文复制引用
刘幸乐,姚继明,吴远明..酸的种类、pH和温度对酸性纤维素酶活力的影响[J].印染助剂,2012,29(12):11-14,4.基金项目
石家庄市科技局科技支撑项目(11119661A) （11119661A）
