医药导报2012，Vol.31Issue(11)：1485-1487,3.DOI:10.3870/yydb.2012.11.031
高效液相色谱法测定盐酸金霉素溶液含量及有关物质
Determination of Content and Related Substances of Chlortetracycline Hydrochloride Solution by HPLC
马永贵 1李自明 1汪秋兰 1贺国芳1
作者信息
- 1. 华中科技大学同济医学院附属同济医院药学部,武汉430030
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determination of the content and related substances of chlortetracycline hydrochloride solution. Methods The HPLC system consisted of C18 column (pH>8, 4.6x250 mm, 5 μm) , 0. 05 mol · L-1 ammonium oxalate solution-dimethylformamide-O. 1 mol · L-1 ammonium dibasic phosphate solution ( 56 : 40 : 4) ( pH being adjusted to 8. 3 with ammonia solution) as mobile phase, the flow rate was 0. 9 mL o min1. Detection wavelength was set at 370nm, the injection volume was 10 uX and column temperature was 40℃. Results The calibration curve was linear in the range of 0. 006 ～ 0. 096 mg o mL-1 (r = 0. 999 8) , the average recoveries were 100. 5% ( RSD = 0.36%),98.7% (RSD=1.04%), 100.9% (RSD = 0.48%) (n = 3), respectively. The limit of detection was 38. 2 ng. Conclusion The method was rapid, simple, accurate, sensitive and suitable for the content and related substances determination of chlortetracycline hydrochloride solution%关键词
金霉素,盐酸/含量测定/有关物质/色谱法,高效液相Key words
Chlortetracycline hydrochloride solution/ Determination of content/ Related substances/ HPLC/ Chromatography分类
医药卫生引用本文复制引用
马永贵,李自明,汪秋兰,贺国芳..高效液相色谱法测定盐酸金霉素溶液含量及有关物质[J].医药导报,2012,31(11):1485-1487,3.
